Welcome to Caltech Life ScienceCaltech Life Science deals with a new revolutionary award-winning green technology to produce recombinant proteins. This is a solid-media based E. coli platform that does not need a fermenter to produce large quantities of biologically active recombinant protein. Caltech works in collaboration with a US based company to provide the recombinant biologics with high yield in a quick time but at a lower cost. This technology is recognized with Frost & Sullivan Award for Technology Innovation for development of a new and innovative protein expression platform.
Protein A binds to the Fc region of immunoglobulins through interactions with heavy chain. The binding of Protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A agarose has been used as a powerful tool to isolate and purify classes, subclasses, and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen. Hence, Protein A agarose is useful for isolating immune complexes
Protein G binds specifically to the Fc region of IgG, strong affinity to monoclonal mouse IgG 1 and rat IgG. It can be used to detect, quantify and purify IgG anti - bodies & antibody/antigen complexes. MPT’s recombinant Protein G, containing 195 amino acid residues which is amino acid 190 - 384 of the Streptococcus sp h as predicated molecular mass of approximately 22.5 kDa. Protein G coupled to Agarose, create extremely useful, easy to use media for many routine applications.
The recombinant Protein A/G fusion comprises a total of 7 IgG - binding domains EDABC - C2C3, which relates to the Protein A and Protein G domains that are contained in the recombinant sequence. The comprehensive Fc - binding properties of Protein A/G fusion make it a standard tool in the study and purification of immu noglobulins. Protein A/G fusion is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins.